Confirmation of 17b-methyl-5b-androst-1-ene-3a,17a-diol metabolite of methandienone in urine by triple quadrupole mass-selective detector gas chromatography (GC/MS/MS) method

  • U. Shorabekova al-Farabi Kazakh National University
  • K. Balgimbekova Anti-Doping Laboratory of Sportsmen, Almaty
  • Aiman Shaldybayeva al-Farabi Kazakh National University
  • Talgat Talbayev Anti-Doping Laboratory of Sportsmen, Almaty
Keywords: anabolic substances, epimetendiol, urine, gas chromatography, doping

Abstract

This article shows the sample preparation used in the analysis, consisting of cleaning of the urine from organic and inorganic chemicals, hydrolysis with β-glucuronidase, extraction with n-pentane and derivatization. Following equipment was used during the performance of this work: a gas chromatograph Agilent Technologies 7890A GC System with 7693 Autosampler and mass selective detector Triple Quad 7000 GC/MS. GC/MS/MS chromatograms of epimetendiol obtained by this method were found to be appropriate. It is shown that the retention time and piek of epimetendiol is 11.29 minute. This method allows confirming epimetendiol's urinary concentration of 1-2 ng/mL.

References

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5 WADA Technical Document – TD2013MRPL Minimum required performance levels for detection and identification of non-threshold substance.
Published
2013-05-14
How to Cite
Shorabekova, U., Balgimbekova, K., Shaldybayeva, A., & Talbayev, T. (2013). Confirmation of 17b-methyl-5b-androst-1-ene-3a,17a-diol metabolite of methandienone in urine by triple quadrupole mass-selective detector gas chromatography (GC/MS/MS) method. Chemical Bulletin of Kazakh National University, 69(1), 44-50. https://doi.org/https://doi.org/10.15328/chemb_2013_144-50