Confirmation of 17b-methyl-5b-androst-1-ene-3a,17a-diol metabolite of methandienone in urine by triple quadrupole mass-selective detector gas chromatography (GC/MS/MS) method
Abstract
This article shows the sample preparation used in the analysis, consisting of cleaning of the urine from organic and inorganic chemicals, hydrolysis with β-glucuronidase, extraction with n-pentane and derivatization. Following equipment was used during the performance of this work: a gas chromatograph Agilent Technologies 7890A GC System with 7693 Autosampler and mass selective detector Triple Quad 7000 GC/MS. GC/MS/MS chromatograms of epimetendiol obtained by this method were found to be appropriate. It is shown that the retention time and piek of epimetendiol is 11.29 minute. This method allows confirming epimetendiol's urinary concentration of 1-2 ng/mL.References
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